The presence of Müller glia, astrocytes, and endothelial cells of blood vessels in the retina containing photoreceptor-like (PR-L) cells in the subretinal space.

A–D: Bright-field view (A), DAPI staining of the nuclei (B), and double-immunostaining for Müller proteins GS (C) and CRALBP (D) of a cross-section of the eye from a 2.5-month-old P_RPE65-ngn3 mouse. E–H: Bright-field view (E), DAPI staining of the nuclei (F), and double-immunostaining for astrocyte protein GFAP (G) and endothelia cells (Endoth) with A10-33/1 (H) of a cross-section of the eye from a 5-month-old P_RPE65-ngn3 mouse. Scale bar, 50 μm, applies to all panels.