The mts^XE2258 allele partially rescues Tap42^RNAi-induced thorax and wing phenotypes.

Panel A: Mitosis and apoptosis in wing discs were monitored using a phospho-Histone H3 (p-H3, green) antibody and TUNEL staining (red), respectively. Control wing discs exhibited phospho-Histone expression throughout the wing disc (A1) with sporadic apoptotic signals (A3). Tap42^RNAi under the control of the ap driver arrested mitosis in the notum area (red dashed line, A2) and triggered massive apoptosis, especially within the wing compartment (red dashed line, A4). Genotypes: (A1 & A3) UAS-Tap42^RNAi/+ as control. (A2 & A4) ap-Gal4/UAS-Tap42^RNAi; +/+. Panel B: Adult control flies (UAS-Tap42^RNAi; mts^XE2258), as well as flies harboring the mts^XE2258 allele alone, did not exhibit any noticeable defect in the thorax (B1 & B3) or wings (B4 & B6). Introduction of the mts^XE2258 allele into the Tap42^RNAi background resulted in a milder thorax cleft phenotype as compared to flies lacking the mts^XE2258 allele (compare B2 with Figs. 1-B3 & 6-B1). Furthermore, the presence of the mts^XE2258 allele resulted in a more developed wing (compare B5 with Fig. 1-C3). Genotypes: (B1 & B4) UAS-Tap42^RNAi, mts^XE2258/CyO. (B2 & B5) ap-Gal4/UAS-Tap42^RNAi, mts^XE2258. (B3 & B6) mts^XE2258/+.