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The most important differences between the cloning experiments performed herein.

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posted on 2015-05-15, 04:17 authored by Lukasz Michal Szafron, Anna Balcerak, Ewa Anna Grzybowska, Barbara Pienkowska-Grela, Anna Felisiak-Golabek, Agnieszka Podgorska, Magdalena Kulesza, Natalia Nowak, Pawel Pomorski, Juliusz Wysocki, Tymon Rubel, Agnieszka Dansonka-Mieszkowska, Bozena Konopka, Martyna Lukasik, Jolanta Kupryjanczyk

1) PCR products shown in this table were first cloned into the pGEM-T Easy vector and then subcloned into one of two target expression vectors listed above. This approach facilitated the cleavage of DNA inserts with restriction enzymes.

2) The pCR3-FL2-CRNDEP plasmid was created by subcloning the CRNDEP insert from the pEGFP-N1_CRNDEP plasmid (without PCR reactions); N/A—not applicable.

The most important differences between the cloning experiments performed herein.

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