Table_2.xls (5.5 kB)
The most important differences between the cloning experiments performed herein.
dataset
posted on 2015-05-15, 04:17 authored by Lukasz Michal Szafron, Anna Balcerak, Ewa Anna Grzybowska, Barbara Pienkowska-Grela, Anna Felisiak-Golabek, Agnieszka Podgorska, Magdalena Kulesza, Natalia Nowak, Pawel Pomorski, Juliusz Wysocki, Tymon Rubel, Agnieszka Dansonka-Mieszkowska, Bozena Konopka, Martyna Lukasik, Jolanta Kupryjanczyk1) PCR products shown in this table were first cloned into the pGEM-T Easy vector and then subcloned into one of two target expression vectors listed above. This approach facilitated the cleavage of DNA inserts with restriction enzymes.
2) The pCR3-FL2-CRNDEP plasmid was created by subcloning the CRNDEP insert from the pEGFP-N1_CRNDEP plasmid (without PCR reactions); N/A—not applicable.
The most important differences between the cloning experiments performed herein.