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The metabolic effects of osteopontin-c depend on the splice junction.

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posted on 2014-08-26, 07:23 authored by Zhanquan Shi, Bo Wang, Tafadzwa Chihanga, Michael A. Kennedy, Georg F. Weber

The metabolic effects of osteopontin-c depend on the splice junction. We stably transfected MCF-7 cells with OPNc versus OPNcM3. Expression was confirmed by Western blotting [44]. MCF-7 cells transfected with osteopontin-c or its mutant M3 were plated in poly-HEMA coated wells. After 48 hours, the cells were harvested and lysed for analysis of the metabolite levels by NMR. Shown are the fold-change and p-value for the steady state-levels of metabolites in osteopontin-c transfectants (C) versus mutant transfectants (M3).

The metabolic effects of osteopontin-c depend on the splice junction.

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