The metabolic effects of osteopontin-c depend on the splice junction.

<p>The metabolic effects of osteopontin-c depend on the splice junction. We stably transfected MCF-7 cells with OPNc versus OPNcM3. Expression was confirmed by Western blotting <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0105675#pone.0105675-Shen1" target="_blank">[44]</a>. MCF-7 cells transfected with osteopontin-c or its mutant M3 were plated in poly-HEMA coated wells. After 48 hours, the cells were harvested and lysed for analysis of the metabolite levels by NMR. Shown are the fold-change and p-value for the steady state-levels of metabolites in osteopontin-c transfectants (C) versus mutant transfectants (M3).</p><p>The metabolic effects of osteopontin-c depend on the splice junction.</p>