The majority of the Sp2 binding sites are also bound by the heterotrimeric transcription factor Nf-y.

<p>Binding sites of the trimeric transcription factor Nf-y were determined by ChIP-seq of the Nf-ya, Nf-yb and Nf-yc subunits using wt and <i>Sp2ko</i> MEFs. (A) Venn diagrams showing the overlap of high-confidence Nf-ya, Nf-yb and Nf-yc binding sites in wild type and in <i>Sp2ko</i> MEFs. (B) Venn diagram showing the overlap of Nf-y and Sp2 binding sites. (C) The strength of Nf-y binding correlates with the strength of Sp2 binding at shared sites. Normalized ChIP-seq tag counts at individual Nf-ya and Sp2 peaks were plotted against each other. (D) Representative genome browser snapshots of promoters bound by Sp2 as well as by Nf-y (<i>Mcm3</i> and <i>Pan2</i>), only by Nf-y (<i>Wapal</i> and <i>Atxn3</i>) or only by Sp2 (<i>Fanci</i> and <i>Taf1c</i>). (E) Sequence motifs enriched at Sp2 sites also bound by Nf-y (left), at sites only bound by Nf-y (middle) or only bound by Sp2 (right). The numbers next to the logos indicate the occurrence of the motifs, and the statistical significance (<i>E</i>-value) [<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005102#pgen.1005102.ref048" target="_blank">48</a>]. (F) Central motif enrichment analysis of the motifs shown in Fig. 7E. (G) Venn diagram showing the overlap of Sp1/3, Nf-y and Sp2 binding sites. (H) Left, sequence motifs at sites that are bound by Sp1/3 but not by Sp2 and Nf-y. Right, sequence motifs at sites that are bound by Sp2 and Sp1/3 but not by Nf-y.</p>