The levels of BSP and integrins.
(A) Flow cytometry (FCM) analyses of αvβ3 protein in the 231BO-BSP27 and 23BO-Scrambled cells, with their negative control in front. The αvβ3 level is lower in the 231-BSP27 cells than in the 231BO-Scrambled cells. (B) FCM analyses for EGFP are used to detect the percentage of the pIRES2-EGFP and pIRES2-hBSP-EGFP transfected cells, while FCM analyses for αvβ3 detect the effect of BSP on the αvβ3 expression. The αvβ3 level is decreased in BSP-depleted cells but is restored by transfection with the pIRES2-hBSP-EGFP. (C) Western blots show that the β3 level is decreased in BSP-depleted cells but is restored by BSP re-expression, with GAPDH as the loading control. (D) Comparisons of the αvβ3, β3 and BSP levels in four cell clones. **p<0.001 vs 231BO-Scrambled.