The impact of biocide exposure on growth.
Panel A. Growth kinetics of SL1344 and cells sorted from exposure to a low dose of biocide concentration. The cells selected from Superkill show a slightly increased lag phase but grow to the same final OD600 as the WT. Lines show a mean of 3 technical replicates and are representative of the cells isolated from each biocide. Error bars are standard deviations of the technical replicates. SL1344 is shown by a continuous line, Superkill by a circle, AQAS by a triangle, Virkon by a square and Trigene by a cross. Panel B. Growth kinetics of SL1344 and mutants sorted from high-level biocide concentrations. The mutants have a much longer lag phase and fail to reach the same final OD600 as the WT. Lines show a mean of 3 biological replicates with the standard deviation. SL1344 is shown by a continuous line, SK 1% by a circle and TR 1% by a square.