The images show that Cytochalasin B affects cellular structure and morphology and disrupts the actin cytoskeleton.

<p>Analysis of HeLa cells after 96 h of treatment. (A) Representative actin stained images of DMSO control cells (left) and 0.6 µM Cyt B-treated cells (right). B) Quantification of cellular spread area (number of cells, DMSO, n = 63; Cyt B, n = 153). (C) Quantification of cellular aspect ratio (number of cells, DMSO, n = 63; Cyt B, n = 153). (D) Cellular mean traction forces measured in 10 kPa polyacrylamide gels (number of cells, DMSO n = 79; Cyt B n = 40). Scale bar is 20 µm. ***: p<0.001.</p>