Figure_1.tif (2.31 MB)
The images show that Cytochalasin B affects cellular structure and morphology and disrupts the actin cytoskeleton.
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posted on 2014-09-30, 02:59 authored by Alberto Elosegui-Artola, Alvaro Jorge-Peñas, Oihana Moreno-Arotzena, Amaia Oregi, Marta Lasa, José Manuel García-Aznar, Elena M. De Juan-Pardo, Rafael AldabeAnalysis of HeLa cells after 96 h of treatment. (A) Representative actin stained images of DMSO control cells (left) and 0.6 µM Cyt B-treated cells (right). B) Quantification of cellular spread area (number of cells, DMSO, n = 63; Cyt B, n = 153). (C) Quantification of cellular aspect ratio (number of cells, DMSO, n = 63; Cyt B, n = 153). (D) Cellular mean traction forces measured in 10 kPa polyacrylamide gels (number of cells, DMSO n = 79; Cyt B n = 40). Scale bar is 20 µm. ***: p<0.001.
