Fig_6.tif (730.57 kB)
The hRpn2-derived peptide depletes hRpn13 from the proteasome.
figure
posted on 2015-10-14, 03:00 authored by Xiuxiu Lu, Fen Liu, Sarah E. Durham, Sergey G. Tarasov, Kylie J. Walters(A) 293T cells were transfected with p3XFLAG-CMV7.1-hRpn2 (916–953) WT or F948Stop plasmids and the cell lysates immunoprecipitated with anti-hRpt3 antibodies and immunoprobed with antibodies against hRpn13, hRpt3, and hRpn2, as indicated. (B) Cell lysates from 293T cells transfected with HA-ubiquitin alone or together with either p3XFLAG-CMV7.1-hRpn2 (916–953) WT or F948Stop plasmids were immunoblotted with anti-ubiquitin and anti-β-actin antibodies, as indicated. (C) A model illustrating hRpn2 (916–953) peptide interacting with hRpn13 and displacing it from the proteasome.