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The gelatinase activity alterations of MMP-2/9 in both cells were assessed by a gelatin zymography assay.

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posted on 2013-06-20, 01:49 authored by Wenjing Zhao, Dan Li, Zuojia Liu, Xiliang Zheng, Jin Wang, Erkang Wang

Gelatin zymography of both CFPAC-1 and MIA PaCa-2 cells was carried out by subjecting conditioned media samples to 10% SDS-PAGE containing 1 mg/mL of gelatin and then stained with 0.25% Coomassie brilliant blue R-250.

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