The expression profiles of OCT-3/4, Brachyury T, SOX-17 and NKX2.5 in END-2 co-cultures.

A) The expression of OCT-3/4 in END-2 co-cultures originating from Matrigel decreased slower than in MEF and SNL feeder cell layer. B) The expression of Brachyury T peaked on day 3 in END-2 co-cultures originating from MEF and SNL feeder cell layers, while from Matrigel the peak was delayed to day 6. C) The expression of SOX-17 behaved in the same way than Brachyury T expression. SOX-17 peaked on day 3 in END-2 co-cultures originating from MEF and SNL feeders, while in co-cultures originating from Matrigel, the SOX-17 peak was delayed to day 6. D) The expression of NKX2.5 was highest on MEF feeder cell layers in the end of END-2 co-culture. The data is collected from two individual differentiation experiments of H7, UTA.00112.hFF and UTA.04602.WT hPSC lines (n = 6 in all three conditions). Error bars show the standard error of the mean (SEM). ** p<0.01, * p<0.05.