The effects of overnight shipping on lymphocyte subpopulations.

<p>PBMC were analyzed post cryo preservation by flow cytometry, to determine the <i>ex vivo</i> frequencies of CD3<sup>+</sup>CD4<sup>+</sup> T cell, CD3<sup>+</sup>CD8<sup>+</sup> T cell, CD3<sup>+</sup>CD127<sup>−</sup>CD25<sup>+</sup>CD4<sup>+</sup> regulatory T cell, CD3<sup>−</sup>CD19<sup>+</sup> B cell and CD3<sup>−</sup> CD56<sup>+</sup> NK cell populations. A) The gating strategy is presented on dot plots from one representative donor. Abbreviations: EM-effector memory cells, CM-central memory cells B) Bar graphs indicate the average frequencies for all tested donors (n = 9) in non-shipped vs. shipped samples. All graphs are shown with SEM values. Before-after graphs show the same data broken down for each individual.</p>