The effects of overnight shipping on lymphocyte subpopulations.

PBMC were analyzed post cryo preservation by flow cytometry, to determine the ex vivo frequencies of CD3+CD4+ T cell, CD3+CD8+ T cell, CD3+CD127CD25+CD4+ regulatory T cell, CD3CD19+ B cell and CD3 CD56+ NK cell populations. A) The gating strategy is presented on dot plots from one representative donor. Abbreviations: EM-effector memory cells, CM-central memory cells B) Bar graphs indicate the average frequencies for all tested donors (n = 9) in non-shipped vs. shipped samples. All graphs are shown with SEM values. Before-after graphs show the same data broken down for each individual.