The effects of overnight shipping on cytokine production.

PBMC were thawed and in vitro stimulated (anti-CD3 + anti-CD28) for six days. Supernatants were collected from the plate-wells and cytokine levels were determined with cytokine bead array for all donors (n = 9). Bar graphs show unstimulated and stimulated conditions both for non-shipped and shipped samples. All graphs are shown with SEM values. Before-after graphs visualize the cytokine levels on individual level. A) Th1/Th2 cytokines; B) Th17 cytokines.