The effect of inhibitors of active cellular transport on the localization of glucose-coated nanoparticles in hCMEC/D3 cells.

(a) Cells were treated with 10 µg/ml nystatin, 5 µg/ml cytochalasin-D, 5 µg/ml nocodazole, 10 µg/ml chlorpromazine, 5 µg/ml cytochalasin-B. Data are expressed as the number of nanoparticles located below the basal membrane compared with untreated cells. Values are the mean ± SEM of at least 50 cells. Anova indicates no significant difference between treatments. (b) Localization of nanoparticles in hCMEC/D3 cells at 8 hours after application following incubation at 37°C or 30°C. U.M. = upper (apical) membrane, Cyt. = cytoplasmic, Ves. = vesicular, L.M. = lower (basal) membrane. The values are the mean ± SEM from at least 50 TEM images from a representative experiment. Data was analysed by Anova; there was no significant difference between the control and antibiotic treated samples (P = 0.703).