The effect of gp120 and Nef on various cytokines (MIP- 1alpha, MIP- 1beta, IL-6, IL-10 and TNF- alpha) in a dose and time dependent manner.

<p>(<b>A</b>) The immDCs were incubated in presence of different concentrations of Nef with 0 ng/ml, 1 ng/ml, 25 ng/ml, 50 ng/ml, 75 ng/ml, 100 ng/ml and 125 ng/ml. The supernatant was collected after 24 hrs followed by measurement of cytokine concentrations through ELISA. Data represent the mean±SEM (n = 3). Statistical analysis was performed using Student's t-test, with the levels of significance defined as p*<0.05 and p**<0.01. (<b>B</b>) The immDCs were incubated in presence of Nef (100 ng/ml) for upto 24 hrs time. The supernatant was collected and the cytokine concentrations were detected through ELISA. Data represent the mean±SEM (n = 3). Statistical analysis was performed using Student's t-test, with the levels of significance defined as p*<0.05. (<b>C</b>) The immDCs were incubated in presence of different concentrations of gp120 (0 µg/ml, 1 µg/ml, 2 µg/ml, 3 µg/ml, 4 µg/ml and 5 µg/ml). The supernatant was collected after 24 hrs followed by measurement of cytokine concentrations through ELISA. Data represent the mean±SEM (n = 3). Statistical analysis was performed using Student's t-test, with the levels of significance defined as p*<0.05 and p**<0.01. (<b>D</b>) The immDCs were incubated in presence of gp120 (4 µg/ml) for upto 24 hrs time. The supernatant was collected and the cytokine concentrations were detected through ELISA. Data represent the mean±SEM (n = 3). Statistical analysis was performed using Student's t-test, with the levels of significance defined as p*<0.05.</p>