The effect of SOD3 on Erk1/2 signaling pathway.

(A) Western blot analysis showed increased Erk1/2 phosphorylation in sod3 transduced muscles at 3-day, 7-day, and 10-day time points (p<0.001 (***), p<0.05 (*), and p<0.001 (***), respectively) as compared to corresponding lacz controls. The samples were normalized with total Erk1/2 antibody. Erk1/2 activation was supported by significant up-regulation of its downstream transcription factors (B) elk-1 (p<0.01 (**)) and (C) ets-1 (p<0.001 (***)), measured by mRNA synthesis in sod3 animals as compared to LacZ control tissues. The injury itself moderately increased production of both molecules as compared to normal uninjured tissue control. However, the significant difference between normal control tissue and LacZ control (p<0.05 (*)) was achieved only in ets-1 expression. (D) Sod3 transduced muscles have increased levels of Akt phosphorylation only at 3-day time point (p<0.001 (***)). The samples were normalized with total Akt antibody.