The characteristics of stromal fibroblasts after treatment with culture media from HeLa cells (HeLa CM) and TGF-β1.

The level of transcriptional change was examined by real-time RT-PCR. A: the expression of miR-21 was significantly up-regulated at 24 and 48 h after HeLa CM or TGF-β1 treatment. B and C: the expression of IL-6 and alpha-SMA was significantly up-regulated at 48 h after HeLa CM or TGF-β1 treatment. D: PDCD4 was significantly down-regulated at 48 h after HeLa CM or TGF-β1 treatment. (*: significant differences from control at P<0.05)