The average growth of <i>E. coli</i> containing various AlaAT and GGAT enzymes.
2013-02-07T00:50:27Z (GMT) by
<p>TR61 <i>E. coli</i> were cultured in a M63 revised minimal medium and expression of AlaAT or GGAT was induced by the addition of arabinose. The average optical density at 600 nm was measured and recorded over a 26 hour time period and is representative of trials done in triplicate. Error bars show standard error, where they exceed symbol size. TR61 cells containing no pBAD vector, cells containing a pBAD vector with no <i>AlaAT</i> or <i>GGAT</i> insert and uninduced TR61 cells containing a pBAD::HvAlaAT enzyme were utilized as controls. <i>E. coli</i> expressing <i>AtAlaAT2</i>, <i>MtAlaAT</i>, <i>ScAlaAT</i> and <i>MmAlaAT</i> showed changes in optical density over the 26 hour time period similar to that seen by <i>Arabidopsis thaliana AlaAT1</i> expressing cells.</p>