The N-terminal 66 residues of Vps74p are not required for <i>cdc34-2</i>-dependent apical growth.
2013-09-03T02:13:10Z (GMT) by
<p>(A) Morphology transformants carrying the plasmid pVT101U (Vec) in <i>cdc34-2</i> mutant were detected. Transformed cells were grown in a synthetic selection medium at 25°C for 2 h and then shifted to 37°C for 6 h. Next, the ability to complement the elongating morphology was investigated using microscopy. Cells of <i>cdc34-2</i> with <i>VPS74</i> deleted were transformed with the indicated plasmids and then imaged as previously described. (B) Yeast strains <i>cdc34-2</i>, <i>pre1-1/pre4-1</i>, <i>cdc53-1</i>, <i>cdc4</i>-, <i>JY25</i>, <i>cdc34-2/vps74</i>Δ<i>, pre1-1/pre4-1/vps74</i>Δ<i>, cdc53-1/vps74</i>Δ<i>, cdc4-/vps74</i>Δ, and <i>JY25/vps74</i>Δ were grown to mid-log phase and these cultures were then transferred from room temperature to 37°C for 6 h and fixed at 37°C. The morphologies of these cells were visualized using microscopy.</p>