The Mediator subunit MDT-15 acts downstream of the p38 MAP kinase PMK-1 to regulate the induction of <i>F08G5.6</i> and <i>F35E12.5</i>.

<p>(A) Wild-type or <i>mdt-15(tm2182)</i> mutant synchronized L1 animals containing the <i>pF08G5.6::GFP</i> immune reporter were grown on vector control (L4440), <i>vhp-1</i>(RNAi) or a combination of <i>vhp-1(RNAi)</i> and <i>pmk-1(RNAi)</i> bacteria and then transferred as L4 animals to PA14 for 18 hours. Animals were photographed under the same imaging conditions. (B) qRT-PCR was used to examine the expression levels of <i>F08G5.6</i>, <i>F35E12.5</i> and <i>C32H11.1</i> in wild-type N2 and <i>mdt-15(tm2182)</i> mutant animals exposed to <i>vhp-1(RNAi)</i> or the vector control (L4440) under basal conditions (as described above) and 8 hours after exposure to <i>P. aeruginosa</i>. Knockdown of <i>vhp-1</i> caused significant induction of <i>F08G5.6</i> and <i>F35E12.5</i> in wild-type N2 animals (<i>p</i><0.001), but not in <i>mdt-15(tm2182)</i> animals (<i>p</i>>0.05), under baseline (<i>E. coli</i>) and pathogen-induced conditions. The expression of <i>C32H11.1</i> was significantly induced by <i>vhp-1(RNAi)</i> (<i>p</i><0.001) in an <i>mdt-15</i>-dependent manner under baseline conditions (<i>p</i><0.001), but not following exposure to <i>P. aeruginosa</i>. Data are the average of two biological replicates each normalized to a control gene with error bars representing SEM and are presented as the value relative to the average expression of the indicated gene in the baseline condition (L4440 animals exposed to <i>E. coli</i>).</p>