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The GFP-ML1Nx2 probe does not dissociate from the Rab5-positive membranes in response to depletion of PtdIns3P and PdIns(3,5)P2.

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posted on 2015-10-13, 03:18 authored by Gerald R. V. Hammond, Shunsuke Takasuga, Takehiko Sasaki, Tamas Balla

Images show a representative cell expressing the three indicated constructs before and 40 min after treatment with 1 μM rapamycin, which induces recruitment of FKBP-MTM1 to the FRB-Rab5-decorated membranes, thereby depleting PtdIns3P and PtdIns(3,5)P2. The graph at right shows mean fluorescence intensity at Rab5-positive membranes normalized to the whole cell for the indicated construct (data are means ± s.e.m. of 15 cells from four independent experiments). No dissociation of the GFP-ML1Nx2 is observed despite robust depletion of FYVE-EEA1 within 2 min of rapamycin application. Scale bar = 15 μm.

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