Figure_3.tif (2.07 MB)
The Ago3-Siwi signal in nuage-like granules increased under the HP1a or -b knocked-down conditions in the silkworm cultured cells.
figure
posted on 2014-03-17, 03:13 authored by Tsuneyuki Tatsuke, Li Zhu, Zhiqing Li, Hitoshi Mitsunobu, Kaito Yoshimura, Hiroaki Mon, Jae Man Lee, Takahiro KusakabeThe BiFC analyses for the interaction between Ago3 and Siwi proteins in each HP1-gene-knockdown BmN4-SID1 cell. The BiFC analyses were performed at 72 h after the introduction of dsHP1a, dsHPb or dsLuc (control). Venus, DsRed and 4′,6-diamidino-2-phenylindole (DAPI) fluorescences indicate Ago3-Siwi, VLG (as a marker for nuage) and the nucleus, respectively. Scale bar: 10 μm.
