The Ago3-Siwi signal in nuage-like granules increased under the HP1a or -b knocked-down conditions in the silkworm cultured cells.
The BiFC analyses for the interaction between Ago3 and Siwi proteins in each HP1-gene-knockdown BmN4-SID1 cell. The BiFC analyses were performed at 72 h after the introduction of dsHP1a, dsHPb or dsLuc (control). Venus, DsRed and 4′,6-diamidino-2-phenylindole (DAPI) fluorescences indicate Ago3-Siwi, VLG (as a marker for nuage) and the nucleus, respectively. Scale bar: 10 μm.