Testing the requirements for Rad60 dimerisation.

<p>A. wt and <i>rad60-SLD2Δ</i> strains were transformed with pREP41 (41), pREP41-<i>rad60</i> (<i>rad60</i>), pREP41-<i>rad60-SLD2Δ</i> (<i>rad60-SLD2Δ</i>) or pREP41-<i>rad60-SLD1Δ</i> (<i>rad60-SLD1Δ</i>) as indicated. Cells were plated on YEA containing HU and MMS as indicated and incubated at 30°C. B. wt or <i>rad60-1</i> cells were transformed with plasmids as indicated. Top row: cells were plated on YEA and incubated at 23°C, 30°C or 36°C as indicated. Bottom row: cells were plated on YEA containing HU or MMS at the doses stated and incubated at 25°C.</p>