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TcRtv prevents chitinase-mediated degradation of procuticular chitin.

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posted on 2013-01-31, 02:10 authored by Sujata S. Chaudhari, Yasuyuki Arakane, Charles A. Specht, Bernard Moussian, Karl J. Kramer, Subbaratnam Muthukrishnan, Richard W. Beeman

(A) Quantitative analysis of total chitin content of pharate adult insects after dsRNA treatment for TcVer, TcRtv, TcRtv+TcCht-5 and TcCht-5 was performed using a modified Morgan-Elson assay (n = 5). Chitin depletion resulting from TcRtv RNAi was rescued following double RNAi for both TcRtv and TcCht-5. Data are reported as mean ± SE (n = 5 each). Statistical significance was computed with Student's t-test. Means identified by different letters (a and b) are significantly different at P<0.05. (B) Immunohistochemical analysis of TcVer, TcRtv, TcRtv+TcCht-5 and TcCht-5 dsRNA-treated insects revealed rescue of the chitin level upon co-knockdown of TcRtv and TcCht5 (dsTcRtv+dsTcCht-5). Chitin (red), TcRtv (green), DAPI (blue), C, cuticle; E, epithelial cell. Scale bar = 5 µm.

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