Figure_2.tif (1.22 MB)
TcCPR27 and TcCPR18 are abundant in cuticle of the pronotum.
figure
posted on 2012-04-26, 02:02 authored by Yasuyuki Arakane, Joseph Lomakin, Stevin H. Gehrke, Yasuaki Hiromasa, John M. Tomich, Subbaratnam Muthukrishnan, Richard W. Beeman, Karl J. Kramer, Michael R. KanostTo suppress cuticle tanning, dsRNA for TcLac2 (2 ng per insect) was injected into day 0 pupae. Proteins from the pronotum and elytra were extracted from five adults at 0–30 min after eclosion, and the extracts were analyzed by SDS-PAGE (left panel). In the dsVer-treated control animals, the extent of tanning of the pronotum was substantially greater than that of the elytron (right panel). S: PBS homogenate supernatant, P: PBS homogenate pellet.
