TRITC-conjugated lectin staining of brain sections 24h after LPS or saline injection with the associated microvascular skeleton extracted with Angio Tool.

(A) left cortex; sham, (B) left cortex; LPS, (C) right cortex; sham, (D) right cortex; LPS, (E) corpus callosum; sham, (F) corpus callosum; LPS. The average microvessel length in the left cortex of brains injected with LPS (B) is reduced with vessels appearing fragmented compared to the brain injected with saline (A). Bar graphs of total microvessel length (G) and average microvessel length (H) in rat brain sections over the cortex and corpus callosum using the skeleton technique with AngioTool. Note the significant decrease in average microvessel length on the left cortex in brains injected with LPS (* p = 0.0093). Scale bar  = 100 μm.