TMEM45A expression is not essential for keratinization.
(A) Relative quantification of KRT14, KRT10, FLG, LOR, IVL, TGM1, SPINK5 and LCE1B mRNA levels in reconstructed epidermis obtained from human keratinocytes transduced with NT shRNA or TMEM45A shRNA after 11 days of reconstruction. Bars represent 95% confidence intervals. Paired t-test (n = 3). No statistically significant difference was observed. (B) Immunofluorescence detection of KRT14, KRT10, IVL and FLG in reconstructed epidermis after 11 days of reconstruction. Scale bars: 50 μm. (C) Immunofluorescence detection of KRT14, KRT10 and LOR in WT and KO mouse adult ear skin. Scale bars: 25 μm. (B, C) The presented data are representative of the results obtained for at least three independent experiments.