TLR4 deficiency suppressed inflammatory and fibrotic responses in diabetic kidney.

<p>The expression of endogenous TLR ligands was upregulated in WT-DN kidneys and TLR4<sup>−/−</sup>diabetic kidneys at week 6 compared with WT non-diabetic controls. (A). Gene expression of IL-6 (A), TNF-α (B), CCL2 (C), CXCL10 (D), TGF-β (E) and fibronectin (F) were upregulated in WT mice with diabetes, but suppressed in TLR4<sup>−/−</sup> mice with diabetes, as measured by RT-PCR. Data are presented as means ± SD; ** <i>p</i><0.01; *** <i>p</i><0.001. The number of animals per group was described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0097985#pone-0097985-g001" target="_blank">Figure 1</a>.</p>