TLR4 deficiency diminished glomerular injury in DN.

<p>WT diabetic mice showed significant glomerular injury including increased kidney to body weight ratio (A), glomerular volume (B), glomerular cellularity (C, week 24), glomerular mesangial matrix (D & E), decreased podocin staining (F) and WT1<sup>+</sup> podocyte numbers (H & I) compared to non-diabetic WT controls. These were attenuated in TLR4<sup>−/−</sup> diabetic mice. (E) Photomicrographs of representative sections of glomerular changes at 24 weeks on PAS staining (×400 magnification). (G) Representative sections of glomeruli were stained for podocin at week 24, with similar staining intensity evident in non-diabetic WT and TLR4<sup>−/−</sup> mice but reduced staining in diabetic mice with a substantially greater reduction seen in WT versus TLR4<sup>−/−</sup> diabetic mice. (I) Representative sections were stained for WT1 at week 24. The data are presented as the means ± SEM; * <i>p</i><0.05; *** <i>p</i><0.001; *** <i>p</i><0.0001. The number of animals per group was described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0097985#pone-0097985-g001" target="_blank">Figure 1</a>.</p>