Figure_8.tif (4.31 MB)
TLR4−/− and MyD88−/− mice show increased severity of infection with C. difficile infection.
figure
posted on 2011-06-30, 00:20 authored by Anthony Ryan, Mark Lynch, Sinead M. Smith, Sylvie Amu, Hendrik J. Nel, Claire E. McCoy, Jennifer K. Dowling, Eve Draper, Vincent O'Reilly, Ciara McCarthy, Julie O'Brien, Déirdre Ní Eidhin, Mary J. O'Connell, Brian Keogh, Charles O. Morton, Thomas R. Rogers, Padraic G. Fallon, Luke A. O'Neill, Dermot Kelleher, Christine E. LoscherMice were pre-treated with antibiotics and infected with C. difficile (see materials and methods). (A) Change in body weight of groups of mice following infection; 6–8 mice per group, data are expressed as mean ± SEM. (B) CFUs in the cecum on day 3 of infection; 3–4 mice per group, data are expressed as mean ± SEM. (C) Representative hematoxylin and eosin-stained sections of the cecum from uninfected (day 0) mice and from animals 3 and 7 post-infected. Original magnification ×200, scale bar 50 µM. (D) Mean histology scores (range 0–3) of sections of the cecum before (day 0) and 3 and 7 days post-infection. Statistical differences between groups was determined by ANOVA, * p<0.05; ** p<0.01, *** p<0.001.
