TDE2508 complex formation.

(A) The whole cell lysate of T. denticola ATCC 35405 was subjected to Western blot analysis with anti-TDE2508 antiserum. Samples in lanes 1–5 were denatured by heating at 100, 80, 60, 37, and 24°C, respectively for 10 min. (B) T. denticola intact cells in lanes 1–6 were treated with 0, 0, 10, 50, 100, and 200 mM of a chemical cross-linker, respectively. The treated cells were sonicated and denatured by heating at 60°C for lane 1 or 100°C for lanes 2–6 for 10 min, and subjected to Western blot analysis with anti-TDE2508 antiserum. The single and double black arrowheads denote a monomer and a complex form of TDE2508, respectively. M denotes a standard marker.