TBC-8 is exclusively expressed in neurons.

(A) Gene structure of tbc-8. Exons are depicted as black boxes and introns as black lines. The position of the tm3802 deletion is indicated (red line). (B) TBC-8 is evolutionarily conserved. Orthologs of TBC-8 are found in H. sapiens and D. melanogaster. TBC-8 contains two predicted domains, a RUN domain (blue) and a TBC-domain (purple). Percentage similarities for both domains are shown relative to C. elegans TBC-8. For full protein sequences of TBC-8 and its orthologs, see Figure S4. (C) Schematic representation of a construct containing a 2873 bp tbc-8 promoter fused to gfp. This transcriptional reporter construct was injected into wild type worms and gfp expression was imaged in stage three larval (L3) worms by confocal microscopy. tbc-8 expression was observed in the neurons of the head including amphid neurons, the ventral nerve cord (VNC) neurons and in neurons of the tail (phasmid neurons). The right panel shows magnifications of the respective regions. Scale bars represent 50 µm.