TACC3 is overexpressed in cervical cancer cell lines and tissues.

(A) The expression of TACC3 in Ect1/E6E7 (HPV-immortalized ectocervical epithelial), CaSki (HPV-16), C33A (HPV-negative), SiHa (HPV-16) and HeLa (HPV-18) cell lines was determined by western blot analysis. The expression levels were compared to three normal cervix tissues. β-actin was used as a loading control. The intensity of bands was quantified using imageJ software and normalized to β-actin. Data shown are means ± SD of at least three independent experiments. (B) Representative immunohistochemical staining on cervical cancer tissue microarray. Quantitative analysis of cervical cancer tissue microarrays showed that the expression of TACC3 is higher in cervical cancer than in normal cervix, but its expression does not correlate with tumor stage (C) or grade (D). Data shown are means ± SD of at least three independent experiments. *, p<0.05; **, p<0.001.