Synthesis and characterization of ganglioside bead array.

A scheme of conjugation of various gangliosides on Bioplex beads via modified EDC/NHS chemistry (a). Dilution-dependent fluorescent response displayed by ganglioside conjugated beads testing various anti-ganglioside rabbit sera (b). Coupling of gangliosides to beads occurred via secondary amine next to ceramide moiety (c). Ceramidase and lipase treatment of beads negate the reporter fluorescent signal (d). The radical R circled in dashed red signifies the presence of sialic acid groups (one in GK1 and GM2, two in GD1b and none in GA1).