Survival curves for non-stem and CSC-like MDA-MB-453 and MDA-MB-231 cells after irradiation.

<p>(<b>A</b>) Colonies were obtained with non-stem and stem-like MDA-MB-453 cells. Cells were unirradiated (0 Gy) or irradiated (6.25 Gy), trypsinized, counted and plated. Cells were grown for 1–3 weeks and stained with crystal violet. (<b>B, C</b>) Survival curves for non-stem and stem-like MDA-MB-453 (<b>B</b>) and MDA-MB-231 (<b>C</b>) cells were determined after irradiation. Cells were exposed to various doses (1.25 Gy-8.75 Gy) of γ-radiation. Cells were trypsinized, counted and plated. Colony formation was determined 1–3 weeks after irradiation. Error bars represent standard error from the mean for three separate experiments.</p>