Support of B19V infection of CD36<sup>+</sup> EPCs under hypoxia <i>vs.</i> normoxia.

<p>(<b>A</b>) Status of CD36<sup>+</sup> EPCs cultured under hypoxia <i>vs.</i> normoxia. Day 4 HSCs were <i>ex vivo</i> expanded under either hypoxia or normoxia to produce CD36<sup>+</sup> EPCs. At day 8, the cells were profiled by flow cytometry analysis for surface antigens indicated. Numbers shown in each plot are percentage of positive cells. (<b>B&C</b>) B19V infection under hypoxia <i>vs.</i> normoxia. Day 8 CD36<sup>+</sup> EPCs were infected with B19V at an MOI of 5,000 gc/cell. (<b>B</b>) At 48 hrs p.i., immunofluorescence staining was performed using anti-NS1 or anti-11 kDa antiserum. DAPI was used to stain the nucleus. Confocal images were taken at a magnification of 40×(objective lens) using an Eclipse C1 Plus confocal microscope controlled by EZ-C1 software (Nikon). (<b>C</b>) At 48 hrs p.i., mRNA was extracted from infected cells, and the levels of B19V VP2-encoding mRNA per β-actin mRNA were quantified.</p>




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