Summarized GI50 values and growth behavior of cells after HBX treatment.
(A) Chemical structure of the USP7 inhibitor HBX. (B) Several growth inhibitory 50 (GI50) values were determined as described in Materials and Methods and summarized. (C+E) Analysis of the growth behavior of A549 and H1299 cells upon HBX treatment. 1.5×106 cells were seeded 15–20 h before HBX treatment. Cells were treated with HBX or DMSO for a total of 15 h and harvested 24 or 48 hours post mock infection (h p.m.i.) and counted. S.e.m. of at least 3 independent experiments is shown. P-values of unpaired, two-tailed t-tests (*P<0.05, **P<0.01, n.s. = not significant). (D+F) The number of dead cells (trypan blue positive) was divided by the total cell number and then normalized to the number of untreated cells. Trypan blue positive cells were counted after using same experimental conditions as in (C+E).
