Subcellular localisation of Ms1.

<p>A: Immunofluorescent detection of endogenous Ms1 in NRCs. Staining is (left-to-right) DAPI (nuclei, blue), α-actinin (green) and Ms1 (red). B: as A but in ARCs. C: western blot detection of Ms1 in subcellular fractions isolated from ARC and NRC cultures. C = cytoplasm, M = membrane, SN = soluble nuclear, CH = chromatin bound, CS = cytoskeleton. D: Sequence alignment of Ms1 in the region of the nuclear localisation signal (NLS). Phosphorylation sites identified experimentally are indicated by a star [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0144614#pone.0144614.ref041" target="_blank">41</a>]. Colours and the subset of sequences are identical to those in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0144614#pone.0144614.g002" target="_blank">Fig 2B</a>. Note that Ms1 from lower organisms lack this region of the protein.</p>