Study of the possible synergistic effect between aspartyl HIV PI and antifungal compounds against <i>F. pedrosoi</i> development.

<p>In this experiments, conidia (1×10<sup>3</sup> cells) were treated or not with a sub-inhibitory concentration of both PIs (saquinavir and nelfinavir at 25 µM) and antifungal drugs (itraconazol at 0.3 µg/ml and amphotericin B at 3 µg/ml), alone or in combination for 1 hour. Afterward, the conidial cells were washed in PBS and inoculated in a fresh solid Czapek medium to measure the CFU. The values represent the mean±standard deviation of three independent experiments performed in triplicate. Symbol denotes system that had a growth rate significantly different from the control (★, <i>P</i><0.001; Student's t test).</p>