Stopped-flow analysis of GroEL D398A-RW.

<p>A. Analysis of fluorescence intensity changes upon addition of 1<i>black trace</i> indicates GroEL R231W, and the <i>gray trace</i> indicates D398A-RW. B. As in A, but with the duration of observation set to 100 sec. <i>Gray trace</i> indicates D398A-RW with ATP added (as in A), <i>red trace</i> indicates fluorescence changes upon addition of 1 mM AMP-PNP.</p>