Stable cell lines with miR-19a target genes exhibit suppression of cell growth, migration, and invasion.

(A) Cell growth of G418-resistant stable cells of A549 (upper) and LK79 (lower) transfected with miR-19a target cDNA expression vectors or empty vector (Ctrl). Cells were counted using Hoechst 33342 staining at 24, 48, and 72 h. The number of each miR-19a target expression cell was significantly less than control cells at 24, 48, and 72 h. (B) Migration assays used A549 (upper) and LK79 (lower) stable cells with miR-19a target genes. Scale bar, 100 μm. (C) In vitro invasion assay used A549 (upper) and LK79 (lower) stable cells with miR-19a target genes. (D) Average number of invading A549 (left) and LK79 (right) stable cells with miR-19a target genes for the invasion assay shown in (C). **, p < 0.005 using a two-tailed t-test.