Spontaneous Odor Identity Discrimination Requires Synaptic Transmission from MB and Depends on G_αq Signaling

(A−E) Spontaneous odor identity discrimination between MCH and BA was severely disrupted (A) by MB ablation (+HU), (B) by transient block (30 °C) of synaptic transmission from MB in C309/shi^ts, 247/shi^ts, or 201Y/shi^ts flies, (C) by developmental disruption of G_αq in MB of dsG_αq/C309, dsG_αq/247, and dsG_αq/201Y flies, (D) by adult-specific, physiological disruption of G_αq in MB of dsG_αq, GAL80^ts/C309 flies at restrictive (30 °C for 3 d) but not permissive (18 °C for 3 d) temperature, or (E) by developmental disruption of G_αq in MB of dG_q^1F1/C309 or dG_q^1F1/247 flies.

(F−H) Spontaneous odor identity discrimination was not affected (F) when the drl gene was knocked down in MB via RNAi-mediated disruption (T. T., H. Tang, and M. Regulski, unpublished data) in drl^RNAi/C309 or drl^RNAi/247 flies, (G) when G_αs was disrupted in MB of G_αs*/C309, G_αs*/247, or G_αs*/201Y flies, or (H) in rut and dnc mutants. n = 4 PIs for all groups.