Spontaneous Odor Identity Discrimination Requires Synaptic Transmission from MB and Depends on G<sub>αq</sub> Signaling

2007-10-02T01:21:35Z (GMT) by Shouzhen Xia Tim Tully
<div><p>(A−E) Spontaneous odor identity discrimination between MCH and BA was severely disrupted (A) by MB ablation (+HU), (B) by transient block (30 °C) of synaptic transmission from MB in C309/shi<sup>ts</sup>, 247/shi<sup>ts</sup>, or 201Y/shi<sup>ts</sup> flies, (C) by developmental disruption of <i>G<sub>αq</sub></i> in MB of ds<i>G<sub>αq</sub></i>/C309, ds<i>G<sub>αq</sub></i>/247, and ds<i>G<sub>αq</sub></i>/201Y flies, (D) by adult-specific, physiological disruption of <i>G<sub>αq</sub></i> in MB of ds<i>G<sub>αq</sub></i>, GAL80<sup>ts</sup>/C309 flies at restrictive (30 °C for 3 d) but not permissive (18 °C for 3 d) temperature, or (E) by developmental disruption of <i>G<sub>αq</sub></i> in MB of <i>dG<sub>q</sub></i><sup>1F1</sup>/C309 or <i>dG<sub>q</sub></i><sup>1F1</sup>/247 flies.</p> <p>(F−H) Spontaneous odor identity discrimination was not affected (F) when the <i>drl</i> gene was knocked down in MB via RNAi-mediated disruption (T. T., H. Tang, and M. Regulski, unpublished data) in <i>drl</i><sup>RNAi</sup>/C309 or <i>drl</i><sup>RNAi</sup>/247 flies, (G) when <i>G<sub>αs</sub></i> was disrupted in MB of <i>G<sub>αs</sub></i>*/C309, <i>G<sub>αs</sub></i>*/247, or <i>G<sub>αs</sub></i>*/201Y flies, or (H) in <i>rut</i> and <i>dnc</i> mutants. <i>n =</i> 4 PIs for all groups.</p></div>