Spontaneous DNA double-strand break formation is reduced in <i>Rev3l</i> complemented MEFs.
2016-01-05T14:56:07Z (GMT) by
<p>(A) DAPI staining of empty vector (EV)-expressing <i>Rev3l</i><sup>+/Δ</sup>, <i>Rev3l</i><sup>-/Δ</sup>, as well as <i>Rev3l</i><sup>-/Δ</sup> MEFs expressing wild-type or ASM recombinant <i>REV3L</i>; arrows indicate micronuclei, with an enlarged example in the inset. (B) Merged immunofluorescence staining of the same MEFs as in (A) with DAPI (blue), 53BP1 (red) and γ-H2AX (green); foci indicate areas of DNA double-strand breaks. (C) Quantification of percent of nuclei that have associated micronuclei. (D) Quantification of cells with fewer than 3, 3 to 5, 6 to 10 or greater than 10 53BP1 foci (as measured using CellProfiler). The bars are color-coded exactly as in Part C to indicate the genotype of the MEFs (*) p < 0.01. Data represent mean ± SEM.</p>