Specificity of HIF-1α dependent activation of the DDX3 gene.

Stable MCF 10A and MCF 7-HIF-1α shRNA clones were generated. (a) MCF 10A-shHIF-1α and (b) MCF 7-shHIF-1α cells were subjected to 200 µM CoCl₂ for the indicated times. qRT-PCR analysis was performed using specific primers for human DDX3, HIF-1α and HIF-2α. DDX3, HIF-1α, and HIF-2α protein levels in cell lysates of MCF 10A-shHIF-1α (c) and MCF 7-shHIF-1α (d) cells were examined by immunoblots using protein specific antibodies. GAPDH served as a loading control. Error bars represent ±SD.