Spatial relationship between FG and charge clusters.

<p>(A) The FG clusters and charge clusters of an example FG nup sequence. (B) Percent overlap of FG clusters with charge clusters (blue). Almost 80 percent of FG motif cluster regions have zero overlap with clusters of charges. Other percentages of overlap, while strongly in the minority, appear with roughly equal probability. FG nups which have been randomly shuffled (in red) have 4 percent of their FG motif cluster regions completely disjoint from charge clusters while there is a strong tendency for FG clusters to overlap with charge clusters with a most probable frequency overlap at 45 percent. Inset shows statistical significance of degree of overlap for FG nups (top, blue) versus WG/FG control group (bottom, red). Horizontal axis in inset shows the deviation of the percent overlap from the mean value for the randomly shuffled ensemble measured in units of the standard deviation of the random ensemble distribution. (B) Histogram of the topological complexity of FG nups (in blue) and randomly shuffled nups (in red). A majority of FG nups (66%) have a low topological complexity (less than 4) with 34% being purely diblock charge-FG copolymer structure, while randomly shuffled nups have only a small minority (22%) with low topological complexity and only 2% are diblocks. Upper inset shows that 77% of FG nups have a topological complexity which is less than their random ensemble by more than one standard deviation, while the control group shows little deviation from the ensemble (red, lower inset).</p>