Soy-derived BBI and KTI protect synthetic lunasin from in vitro digestion.

The upper, lighter bands are Coomassie Blue protein stains and the lower, darker bands are Western blots. (M) Markers, (1) Lunasin (600 ng) + pancreatin (600 ng) incubated for 0 h at 37°C, (2) Lunasin (600 ng) + pancreatin (600 ng) incubated for 1 h at 37°C, (3) Lunasin (600 ng) + BBI (18,000 ng, unboiled) + pancreatin (600 ng) incubated for 1 h at 37°C, (4). Lunasin (600 ng) + KTI (18,000 ng, unboiled) + pancreatin (600 ng) incubated for 1 h at 37°C, (5) Lunasin (600 ng) + BBI (18,000 ng, boiled) + pancreatin (600 ng) incubated for 1 h at 37°C, (6) Lunasin (600 ng)+KTI (18,000 ng, boiled) + pancreatin (600 ng) incubated for 1 h at 37°C, (7) BBI (unboiled), (8) KTI (unboiled).

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