Southern blot analysis of three T<sub>3</sub> transgenic cotton lines.

<p>Southern blot hybridization analysis of genomic DNA extracted from leaves of three transgenic cotton plants and non-transformed negative control plants. (a) DNA from transgenic cotton plants and non-transformed negative control plants was digested with <i>EcoR</i>I and hybridized with a DIG-labeled <i>p35</i> probe (870 bp); (b) DNA from three transgenic cotton plants and non-transformed negative control plants was digested with <i>EcoR</i>I and hybridized with a DIG-labeled <i>op-iap</i> probe (583 bp); Lane 1 the negative control plant; lanes 2, 3, 4 the three transgenics lines (KB1, KB2, KB3); lane 5: positive control [purified PCR products of the <i>p35</i> (870 bp) and <i>op-iap</i> (583 bp) coding region]; lane M molecular mass marker is GeneRuler TM 1 kb DNA ladder (MBI Fermentas, Maryland, USA).</p>