Figure_5.tif (867.27 kB)
Southern blot analysis of DNA treated with Topoisomerase IV.
figure
posted on 2012-06-18, 00:53 authored by Mari Espelund, Marianne A. Minge, Tove M. Gabrielsen, Alexander J. Nederbragt, Kamran Shalchian-Tabrizi, Christian Otis, Monique Turmel, Claude Lemieux, Kjetill S. JakobsenThe filter contains pooled CsCl fractions of K. veneficum DNA, undigested (−), treated with Topoisomerase IV (To), and digested with EcoRI (E). The hybridization probe is identical to probe 4 of Figure 3, containing the highly conserved motif 2 and a partial sequence of motif 1. The location of the plastid genome is indicated by green shade above the lanes.
