Figure_1.tif (889.89 kB)
SmpB-SsrA Mediated Protein Tagging Activity Is Observed in Y. pseudotuberculosis
figure
posted on 2013-02-22, 08:49 authored by Nihal A Okan, James B Bliska, A. Wali Karzai(A) Schematic representation of the λ-cI-N-trpAt reporter construct encoded on the pKW540 plasmid and anticipated outcomes of protein tagging in wild-type (WT) or ΔBA strains.
(B) The λ-cI-N-trpAt reporter was induced in wild-type and mutant (ΔBA) strains, and protein samples were analyzed by Western blot using HRP-conjugated anti-H6 serum.
MW stds, protein molecular weight standards.