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Skeletal muscle regeneration is impaired by the absence of T cells.

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posted on 2015-06-03, 15:00 authored by Alessandra Castiglioni, Gianfranca Corna, Elena Rigamonti, Veronica Basso, Michela Vezzoli, Antonella Monno, Albert E. Almada, Anna Mondino, Amy J. Wagers, Angelo A. Manfredi, Patrizia Rovere-Querini

Muscle weight (A), cross-sectional area (CSA) of myofibers (B) and the number of satellite cells (C) are comparable in uninjured Rag2-/- γ-chain-/- (black bars) and C57Bl6 (WT, white bars) mice (n = 4 mice per group, mean ± SEM). (D) Representative images of skeletal muscle sections at day 15 after CTX. Centrally nucleated myofibers (new formed fibers) are shown (Red = Laminin; Blue = Hoechst). (E) At day 15 and 20 after injury, the CSA of myofibers is significantly lower in the absence of T lymphocytes (Rag2-/- γ-chain-/- mice, black bars) (n ≥ 2 mice per group, mean ± SEM). (F) Pax7 expression peaks at day 5 is blunted in Rag2-/- γ-chain-/- mice (black circles); the same mice did not show differences in the level of expression of Igf1 compared to WT mice (white circles) (n ≥ 2 mice per group, mean ± SEM; fold increase was calculated with reference to the untreated muscle, NT). (G) Rag2-/- γ-chain-/- mice (black circles) show a blunted expansion of satellite cells at 5 days after injury compared to WT mice (white circles) (n ≥ 3 mice per group, mean ± SEM; fold increase was calculated with reference to the untreated muscle of the corresponding genotype, NT).

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